Studies on Urobilinogen

Studies on the Determination of Bile Pigments. Vi. Urobilinogen in Urine as Urobilinogen-aldehyde.

THE photometric determination of urobilinogen was proposed by Terwen (i) and employs the reaction of native urobilinogen, plus that derived from ferrous hydroxide reduction of urobilin, with Ehrlich’s reagent, p-dimethylaminobenzaldehyde. This basic approach culminated in the “quantitative” and “semiquantitative” methods of Watson (2,3) with minor modifications by various authors (4). Efforts i...

Studies on the determination of bile pigments. III. Standardization of the determination of urobilinogen as urobilinogen-aldehyde.

Several preparations of urobilin and stercobilin were investigated with regard to their value as standards for the determination of urobilinogen and urobilin in biologic specimens. Products of commercial origin were found to be impure and therefore unsuitable for this purpose. Absorptivities were measured for solutions of the pigments in 3% FICI in methanol and for the chromogens, obtained by f...

Cxlvii. Studies on Bile Pigments. Iii. the Quantitative Determination of Urobilin and Urobilinogen in Urine and Faeces

IN a previous paper [Naumann, 1936] the qualitative tests for urobilinogen and urobilin have been studied. In the following the quantitative aspect of the problem is treated. A basis for the estimation of urobilin and related compounds was first introduced by Terwen [1924; 1925]. The main features of this method consist in determining the sum of urobilinogen and urobilin as urobilinogen after r...

The isolation of crystalline d-urobilinogen.

Until recently there have been only two well recognized members of the urobilinogen group, mesobilirubinogen, the chromogen of i-urobilin,’ and stercobilinogen, the leuco compound of urobilin or stercobilin. While mesobilirubinogen is readily obtained in crystalline form following amalgam reduction of bilirubin or i-urobilin, stercobilinogen has never been crystallized. Stercobilin, however, wa...

Increased urinary urobilinogen following acute myocardial infarction.